FDG kinetics in cells and tissues: a biochemically-driven compartmental approach

The radioactive glucose analogue 2-deoxy-2-[F]fluoro-D-glucose (FDG) is widely used to reconstruct glucose metabolism and other biological functions in cells and tissues. The analysis of data on the time course of FDG tracer distribution is performed by the use of appropriate compartmental models. Motivated by recent results in cell biochemistry, we describe a new compartmental model aiming at the reconstruction of tracer kinetics in cells and tissues, which emphasizes the different roles of the cytosol and of the endoplasmic reticulum. Two applications of the new model are examined, that are concerned with real data from cancer cell cultures in vitro, and cancer tissues in vivo. The results are compared with those obtained through application of more standard compartmental models against the same datasets and appear to be in a better agreement with respect to recent biochemical experimental evidence. In particular, it is shown that tracer tends to accumulate in the endoplasmic reticulum, rather than cytosol, and that the rate of phosphorylation is higher than predicted by current models. M. Scussolini Dipartimento di Matematica, Università di Genova, Via Dodecaneso 35, 16146 Genova, Italy E-mail: [email protected] V. Cossu Dipartimento di Medicina Nucleare, IRCCS-IST San Martino, Salita Superiore della Noce 29, 16131 Genova, Italy C. Marini Dipartimento di Medicina Nucleare, IRCCS-IST San Martino, Salita Superiore della Noce 29, 16131 Genova, Italy, and Dipartimento di Scienze della Salute, Università di Genova, Via Antonio Pastore 1 16132, Genova, Italy, and CNR Istituto di Bioimmagini e Fisiologia Molecolare (IBFM), Via Fratelli Cervi 93, 20090 Milano, Italy G. Sambuceti Dipartimento di Medicina Nucleare, IRCCS-IST San Martino, Salita Superiore della Noce 29, 16131 Genova, Italy, and Dipartimento di Scienze della Salute, Università di Genova, Via Antonio Pastore 1, 16132 Genova, Italy G. Caviglia Dipartimento di Matematica, Università di Genova, Via Dodecaneso 35, 16146 Genova, Italy ar X iv :1 80 3. 05 02 5v 1 [ qbi o. T O ] 1 3 M ar 2 01 8 2 Mara Scussolini et al.